Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. 2016;47:15964. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. Biomaterials. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Drug Deliv. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. An undergraduate physical chemistry experiment. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Carbohydrate Polymers 88 1440-1444. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). (2003). Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Smith, David R.; Raymonda, John W. (1972). Macromolecules. J Appl Toxicol. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Journal of Applied Polymer Science, 27, 795-810. Solution properties of branched dextran. (1990). Naik A, Kalia YN, Guy RH. AATEX. (1954). dextran hydrodynamic radius. International Journal of Biological Macromolecules, 23, 135-141. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. 8. Format). Saudi Pharm J. Journal of Chemical Education, 80(9), 1036-1038. Food Sci Biotechnol. Polysaccharide hydrogels for modified release formulations. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Stenekes, R.J.H. Epub 2012 May 28. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. This study was supported by the LG Electronics. Ibrahim O, Munavalli GS, Dover JS. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Radiofrequency with microneedling. Drug Deliv. J. Braz. (2001). The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Crini, G. (2005). J Control Release. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. International Journal of Pharmaceutics, 277, 99-104. ; Wood, P.J. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Intrinsic viscosity of bovine serum albumin conformers. (2007). Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. ; Picout, D.R. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Garcia de la Torre, J.; Carrasco, B. Dextran. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Expert Rev Med Devices. Nat Biotechnol. WebDextran 40 Pharmaceutical Quality. Polymolecularity and polydispersity in Molecular weight determinations. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Ther. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Chemical penetration enhancers for transdermal drug delivery systems. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. The micropore formed at the SC through to the epidermis, enabling drug delivery. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Carbohydrate Polymers 99, 199-207. Sci. 2015;23(3):2239. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. volume24, Articlenumber:22 (2020) Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. The above data are not useful because it can not be compared with those obtained in this work. Springer Editions. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Polymer molecular weight distribution. Provided by the Springer Nature SharedIt content-sharing initiative. The genus Weissella: taxonomy, ecology and biotechnological potential. 2023 BioMed Central Ltd unless otherwise stated. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Thermo Fisher Scientific. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Biotechnology Progress, 19, 544-548. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. ; Garca de , J. Proc of SPIE. Morris, G.A. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Doke SK, Dhawale SC. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. After 24h, the cumulative permeation of FITC-dextran M.W. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Google Scholar. Carbohydrate Polymers, 68, 477-488. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Epub 2012 Oct 18. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). On single point determination of intrinsic viscosity. (2008). The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Probiotics Antimicrob Proteins. All authors have consented to the submission of this manuscript for publication. 2015;32(5):170413. Article Colloid Polym. Ioan, C.E. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Trop J Pharm Res. The hydrodynamic radius of 2-NBDG glucose was too small to measure. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Anal. Chem. This site needs JavaScript to work properly. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Journal of Applied Polymer Science, 13, 2031-2032. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). In addition, the permeation rate was higher in pig skin than in human skin. By using this website, you agree to our The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. European Polymer Journal, 36, 2143-2146. WebTry out PMC Labs and tell us what you think. Pharm Res. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). 2012;27(3):20212. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. of different origins. Gold MH, Biron JA. Int J Food Sci Nutr. Beer, M.U. Progr. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Each microporated sample was incubated in the skin culture medium. Carbohydrate Polymers 88 373-381. (1991). A solvent at this temperature is called a theta solvent [53]. (2013). Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Advances in Physical Chemistry, Vol. Huggins equation [41] gives the relation to the relative viscosity increase (i). (2003). Curley SA. Thus, it is a common practice to express the frictional coefficient of rigid structures as. (2008). official website and that any information you provide is encrypted Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Radiofrequency ablation of malignant liver tumors. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Theory, Estimation, Experiment, and Data. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Differences were considered statistically significant at p <0.05. 4h after microporation, the sizes of the micropore decreased slightly. Sachdeva V, Banga AK. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. WebR H Hydrodynamic radius [m] The basic DLS setup. Die Makromolekulare Chemie, 148(1), 229-238. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Chem. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). monzo closed account refund, holly revord wiki, tambaram police commissioner office address,